Planting Science - Projects: pbchsbartenslagercfernspring2023 project 5
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pbchsbartenslagercfernspring2023 project 5

Project by group pbchsbartenslagercfernspring2023

Explore From my experiences outside of school, I knew that plants reproduced sexually and asexually and some basic concepts of plants. The research done in class expand my knowledge of the classification and characteristics of plants like how the C.fern has no vascular tissue and reproduces with spores. I also learned about the alternation of generations in Ferns. You start with a sporophyte generation that is large and diploid that makes spore using meiosis. Spores are held in sporangia, which are in clusters called sori located under the fronds of the ferns. Those spores are haploid then develop into gametophytes that have male parts named antheridium and female parts called archegonium. The male and female parts produce sperm and egg through mitosis which then join together to produce a zygote using a film of water.
Research Question How do different types of agar affect the germination of the C.fern? The three groups we will be testing are agars with no nutrients, nutrient agar, and Carolina fern nutrient agar.
Predictions The group predicts that the experimental group containing Carolina C.fern nutrient agar will have the highest germination rates. This is because the agar is specifically made to cultivate ferns, so not only will the nutrients be better used but the risk of contamination from bacteria will be lower.
Experimental Design The materials used were: four petri dishes, Carolina nutrient agar, Non-nutrient agar, Nutrient agar, Stirring rod, Hot plate, 10% bleach, C-Fern Carolina Spores, Ziploc bags, Sharpie markers, 250mL beaker, Latex gloves, Spore spreader, Micropipette, and Hot hands The variables were: IV→ Type of agar used, DV→ The rate of germination, Constants→ Amount of water used, amount of agar used, amount of sunlight, amount of spore solution per petri dish, time permitted to grow, temperature for each petri dish, spore spreader, The procedure was as follows: 1. Using 10% bleach, disinfect the area of work. 2. Fill three 250mL beakers with 120mL of water. 3. Label each beaker as “Non-Nutrient,” “Nutrient,” and “Carolina Nutrient.” 4. For the beakers labeled, “Nutrient” and “Carolina Nutrient,” mix the proportional amount of each agar in its corresponding beaker. 5. Place each beaker on a hot plate set to high until the solution boils, mixing with a stirring rod. 6. Take the beakers off the hot plate with hot hands and let them cool for ten minutes. 7. After ten minutes, pour each beaker of agar into four petri dishes using the clamshell technique. 8. Wait for the agar to solidify then close the plate and flip it upside down. 9. Store the plates for 24 hours. 10. After 24 hours, add 100 microliters of C-fern Carolina Spores to each plate using a pipette. 11. Spread the spore solution using a spore spreader. 12. Store plates in a partially closed plastic bag on the windowsill while the spores germinate.
Investigation Theme CFERN
Grade Level High School Students (Grades 9,10,11,12)
Teacher Name William Bartenslager
School Name Palm Beach Central High School
Session Spring 2023


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NSF_Logo.jpg This material is based upon work supported by the National Science Foundation under Grant #2010556 and #1502892. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation.

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