GEHS Beardsley Hr7 Team1

Project by group gehsbeardsleyfall2016project

Info

Explore What we know about plants from outside of school is that they are important for taking carbon dioxide and relasese oxygen as a by product. Though we got most of our information about Arabidopsis thaliana from research in class. This information is summarized on the PowerPoint and our rough draft...
Research Question What genotypes for arabidopsis thaliana are more apt to grow in a human contaminated environment?
Predictions The control experiment will grow normally like in the wild without any added salt, the low concentration will stub the growth of the plant and the high concentration could kill the plant.
Experimental Design We plan to test three strains of Arabidopsis thaliana (2 wild types: Colombian and Landsberg erecta, and a mutant strain of the Colombian) on their response in growth to three different concentrations of salt in the water we use to water them. One pot of each strain will be watered with normal...
Conclusion From our data it is shown that the mutant with the salt sensitive genes can not survive in high salt levels nor in low salt levels. We also found the Landsberg and Columbia strains are also extremely effect by salt levels and could not grow in high salt levels. Landsberg and Columbia strains...

Updates

Get to know your team’s scientist mentor, who will encourage and guide you through the scientific process of discovery. The more you share your ideas and research info, the more your mentor can help. You may also hear from a scientist mentor liaison who will be helping all the teams in your class.
PlantingScience Staff
has been updated by administrator
PlantingScience Staff
uploaded Proposal.pdf in project files
victoria
said

Hello, 

I could not thank you enough for your effort, advice and experience. It was very useful and helpful in improving our experiment. I am honored that we could have such an amazing scientist that took time out of their day and used their expertise to help collaborate with us to make our experiment better. Thank you again.

elizabeth
said

I would like to thank you as well for helping us improve and giving great advice so our experiment could be successful! Thank you for taking time out of your busy schedule to answer our questions!

grace
said

I would also like to thank you so much for helping us with this experiment. You were always quick to answering our questions and having your expertise made our project go by much smoother. Thanks again for all of your help! 

grace
updated the project info
elizabeth
uploaded Experiment Poster.pdf in project files
grace
said

We finished the data and orignally struggled to process the data statistcally using ANOVA using averages. Instead, we took all of the measurements from the last day only and found that we could reject the null hypothesis and that all the data was statisticaly significant. I am going to add our formal conclusion along with our finished poster.  We used a line graph to display our height data and a bar graph to display our percent survival rate. For percent survival rate, because we thinned out the control group once they got too long, we only took data for day 18 because the data would have been skewed from thinning the plants out. 

Julie Ann Herman
said

Hey all!

I imagine you are on break this week, but I'm looking forward to seeing your data analysis. What kinds of graphs do you think would be good to display your data?

elizabeth
said

Experiment Day 28 11/14/16

Today we are completely done with measure the height of all the plants. We started to get out the control, no salt, plants and all but the mutant plants in 150 millimolar of salt for measuring biomass. Due to the roots being too fine,  we are only measuring the biomass of the stems. We are waiting to confirm with our scientist if we should dry out the plants of Saturday and measure the biomass as we are unsure if we need that data as well. We did get the plants out and place them in envelopes to have them gradually dry.

Experiment Day 30  11/16/16

we finished collecting height data and we decided to not measure biomass as our scientist believes it might not be needed, and she suggested to maybe have percent germination or survival percentage. We decided to do survival percentage of the plants to help clearly illustrate our data. We also had to fix the rounding of averages in our height data to the tenths place instead so it is easier to interprete the data. 

 

 

Julie Ann Herman
said

If you think one of your other measurements will show clear differences, don't make your life harder by collecting extra data! I think that is one of the most important lessons you can learn as a scientist :)

Since so many of your high salt plants died, I think one of the ways you can compare all your treatments might be % germination and days of survival. Can you think of any others?

elizabeth
said

Also we are only doing the biomass for the no salt plants and all the plants, expect the mutant( because there was nothing to measure), the in 150 millimolar of salt.

elizabeth
said

Hey so we finished with collecting the height data, but we are unsure if we should still do biomass as all the plants look different enough for good data analysis. We started pulling out the plants, but the roots were to small to pull out, so we were going to start measuring the biomass of the stems and leaves only. We have not started the drying process yet, but we would like to know if we should get this data too.

grace
said

Experiment Day 24

Today we finished measuring the control group. We had started measuring the plants on Day 21, but did not have enough time to finish measuring or recording. Thing are about the same in the high salt environment. No plants are living. The low salt environment also proves to be too much for the plants as only a few plants in the Landsberg and Columbia strains are surviving and green , and there are no plants left in the mutant pots. The controlhave grown very rapidly and are so tall we removed the top of the container. As for the fungus, we noticed it in the High salt environment as well as the low salt environment. we believe the mod grew because of an excess of water combined with the decomposing of the plants. The fungus helped break down the dying plants which couldn't survive the salted environment. 

grace
said

Experiment Day 24

Today we finished measuring the control group. We had started measuring the plants on Day 21, but did not have enough time to finish measuring or recording. Thing are about the same in the high salt environment. No plants are living. The low salt environment also proves to be too much for the plants as only a few plants in the Landsberg and Columbia strains are surviving and green , and there are no plants left in the mutant pots. The controlhave grown very rapidly and are so tall we removed the top of the container. As for the fungus, we noticed it in the High salt environment as well as the low salt environment. we believe the mod grew because of an excess of water combined with the decomposing of the plants. The fungus helped break down the dying plants which couldn't survive the salted environment. 

elizabeth
uploaded Data.pdf in project files
Julie Ann Herman
said

Nice data! Did you note which containers the fungus is growing in? Do you have any ideas why?

elizabeth
uploaded Data.pdf in project files
grace
said

Experiment Day 13  OBSERVATIONS FOR Oct. 31
We did a normal measuring day. All strains of plants in the 300 millimolar solution appear dead; they are brown and withered. The salt sensitive mutant strain in the low salt also appeared to be dead because they were brown and withered. In the low salt the Columbia and Landsberg plants appeared to be around the same heights. All of our plants in the control are green, growing, and thriving. We watered today, but we are planning on leaving them dry the next time.

 

Experiment Day 18 11/4/16
Another measuring day. The plants were left in dry conditions to prevent over saturation. Plants in the control set are continuing to thrive, with a noticeable difference in height, with the Landsberg being the tallest. In the low salt, the mutant still has not grown and some plants appear to be purple. The Columbia and Landsberg are growing but are much farther behind than the control plants in terms of growth stages. The high salt has killed most of the plants, and the ones still there are not growing. There is evidence of fungal activity. We watered the plants again. Also we thinned out the control plants to about three plants per pot to combat competition between plants. We also were able to notice differences between trichome growth in the control plants(Landsberg and Columbia have them while mutant does not). We will watch this in our salt solutions from now on.

 

 

Julie Ann Herman
said

Good call on making sure the plants don't get too salty! I hope they're still doing ok. Looking forward to hearing more progress!

grace
said

Sorry we uploaded  the wrong data charts we will add the new ones. 

Also we are going to update you on our daily experiment work through the updates since files is hard to update regularly:

 

Experiment Day 610/24/16
All plants have germinated and we started to water the plants with the appropriate water solution. We watered 15ml for each plant, then we added additional amount of water in each of the trays. We also started to measure the shoot length of all of the plants that have sprouted.

Experiment Day 7 10/25/16
Although we didn't measure today there was a slight difference in growth, we noticed the Control plants and low salt plants seemed to continue to grow whereas the high salt plants’ growth seemed to be stunted. We also made more salt solution to store away for the next time we may need it. The  solutions are 300 millimolar (17.5 grams of salt per liter) and 150 millimolar (8.7 grams per liter). 

Experiment Day 9 10/27/16
Today we measured the plants again. We have found that the No salt plants have grown and developed much more than the low salt plants, and the high salt plants seemed to not grow at all. The high salt even seemed to be slightly discolored. We ran into a problem with evaporation. The water evaporated from the tray but the salt crystals were left behind. We were worried about adding salty water to already salty trays, se we decided to rinse out the salt from the bottom of the tray. After rinsing out the salt, we added more water to the plants. We also pre made some more solution for the next time that we need to water the plants. 

elizabeth
uploaded Data.pdf in project files
Julie Ann Herman
said

Great! I don't see anything in the data sheet yet... maybe you just haven't uploaded the most recent one? Also, I am not sure I understand how the data sheet is set up--maybe some titles and more labels would help me know what the three different pages are for.

elizabeth
said

Hey so we updated our experiment journal as well as added height numbers  in our data chart. We are measureing in millimeters.

elizabeth
uploaded Experiment Journal.pdf in project files