Forest Rascals

Project by group chsparfittspring2020

Updates

PlantingScience Staff
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Gwen
said

Hey! We communicated with our teacher about using a hemacytometer to count how many spores there are as well as using a smaller amount of water and more dishes. Unfortunately, we don't have those resources. However, we will present our…

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Hey! We communicated with our teacher about using a hemacytometer to count how many spores there are as well as using a smaller amount of water and more dishes. Unfortunately, we don't have those resources. However, we will present our information collected today in another message.

    Elizabeth Stunz
    said

    Hello Gwen!

    Thanks for the update. It sounds like you are able to quantify spores per droplet pretty reliably and you can always create a makeshift hemocytometer with gridded paper under your petri dishes...I'm sure you will come up with…

    more

    Hello Gwen!

    Thanks for the update. It sounds like you are able to quantify spores per droplet pretty reliably and you can always create a makeshift hemocytometer with gridded paper under your petri dishes...I'm sure you will come up with creative, repeatable methods to obtain good data for your project. I look forward to hearing more about your experimental set-up and gametophyte growth as your project progresses!

Avery
changed the project settings
Avery
updated the project info
Elizabeth Stunz
said

Hi team,

Your experimental design looks good! I know you're not sure about spore #s yet, but do you have any estimates about what you might expect per treatment? Also, would you be able to use a hemacytometer to count how many spores per drop…

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Hi team,

Your experimental design looks good! I know you're not sure about spore #s yet, but do you have any estimates about what you might expect per treatment? Also, would you be able to use a hemacytometer to count how many spores per drop there are? A hemocytometer is just a gridded slide to use with a compound microscope to count cells (or in this case spores).

Also, 10mL increments may be a little much, especially depending on how many spores you will have in solution. It may be more appropriate to dilute by 2mL or 5mL per treatment. Also, do you think it would be possible to do one or two more replicates per treatment? If you can only use 4 dishes for your experiment, that's fine, but if you could use 12 (3 replicates per treatment) then you would have much more statistical power and stronger results if you end up seeing similar patterns for all replicates of each treatment.

Your hypotheses about what you may see sound reasonable and well thought-out. I look forward to seeing photos of your experiment once you get it set up!

Gwen
said

our experiment set up below

Gwen
uploaded IMG_9184.jpg in project files
Gwen
said

Our hypothesis-

We expect the male population to increase as there is more room for males to avoid females.

We expect the germination rate to increase with the more room for spores

Gwen
said

We will analyze our spores under a microscope as we will be able to determine the male to female ratio as well as germination rate under a microscope.

 

Avery
said

I think we are going to use 4 dishes to do our experiment with one of those dishes being our control. We were thinking about also doing our dilutions in increments of 10 mL

Gwen
uploaded IMG_8442.jpg in project files
Gwen
said

We created a diagram of our experiment however it is not allowing us to paste or save it.- nevermind, we figured it out

Katie
said

Can you please clarify what an independent and dependent variable so we can apply it to our experiment of serial dilution?

    Elizabeth Stunz
    said

    Hi Katie,

    I think the best way to remember the difference between independent and dependent variables is to think of the independent variable as the manipulated variable and the dependent variable as the response variable. For example, if…

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    Hi Katie,

    I think the best way to remember the difference between independent and dependent variables is to think of the independent variable as the manipulated variable and the dependent variable as the response variable. For example, if you wanted to test how salinity affects plant growth then your saline concentrations would be your manipulated variable and your measurements of plant height and leaf length would be your response variables.

    Hope that's helpful, and let me know if I can clarify anything else.

Gwen
said

Finally, what substrate do you plan to grow your ferns on and how many serial dilutions would you use? What dilutions would you like to test?

So, we are thinking we want to the serial dillution to acess the competition among spores…

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Finally, what substrate do you plan to grow your ferns on and how many serial dilutions would you use? What dilutions would you like to test?

So, we are thinking we want to the serial dillution to acess the competition among spores assosiated with growth rates. We think that we have the most correct materials for this experiment.

    Elizabeth Stunz
    said

    Gwen,

    Those sound like great questions to test. So, you will be inoculating plates with different spore dilutions and then you plan to collect data on how many spores become gametophytes as well as whether gametophytes are…

    more

    Gwen,

    Those sound like great questions to test. So, you will be inoculating plates with different spore dilutions and then you plan to collect data on how many spores become gametophytes as well as whether gametophytes are hermaphrodites or males?

    Also, how many dilution treatments and many replications for each dilution treatment do you plan to use?

Kim Allyn Parfitt
said

Hey Forest Rascals,

I love your enthusiasm and you made great progress today on your scientific thinking related to the Cfern experiment. I do, however, suggest that you up your game related to being scholarly for the Engage part of your…

more

Hey Forest Rascals,

I love your enthusiasm and you made great progress today on your scientific thinking related to the Cfern experiment. I do, however, suggest that you up your game related to being scholarly for the Engage part of your experiment project page. Remember, you are representing Central High School and I know how amazingly fantastic you all are so let's have your work reflect that :)

Kim Allyn Parfitt
joined the project
Gwen
said

Gwen
said

Gwen
changed the project settings
Gwen
updated the project info
Gwen
changed the project settings
Gwen
updated the project info
Gwen
changed the project settings
Gwen
updated the project info
Gwen
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