Planting Science - Projects: whssweetfall2020 project 8
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whssweetfall2020 project 8

Project by group whssweetfall2020

Explore When researching I found that plants' function is to produce oxygen and go through photosynthesis. They receive energy by absorbing light energy within their leaves and taking in carbon dioxide and water to make glucose a sugar. I learned that microbes are pathogens because they both cause harm to their host because they are bacteria or viruses. I also learned that some plants are more tolerant to diseases because they evolve over time rapidly. The environment effects a big part in plants being more tolerant as well as their humidity and the soil type. This impacts plant diseases by their environmental conditions, water availability, and pests. I wonder how people working in the agriculture industry identify when a plant begins to have microbes or pathogens, which is a virus or bacteria that damages the well being of the plant because both pathogens and microbes harm organisms since they are considered diseases or germs.
Research Question What plants are more susceptible to soft rot?
Predictions The middle or the top of the potato will have a brown blackish color and the rest of it will have the same color but softish.
Experimental Design Independent: We will inoculate the soft rot into the potato to see if it succumbs or fails to resist the soft rot. Dependent: We will be measuring the plant detteriation from the soft rot we gave the potato as well as the other 2 vegetables. Control: The potatoes that weren’t affected during the process of the inoculation of the soft rot. Gather all your materials Damp paper towels, Sealable plastic bags, Toothpicks, Small beakers of bacterial inoculum, A 10% bleach solution containing two drops of dish soap, A large container for disinfecting the potatoes in 10% bleach, Microscopy equipment (microscopes with 400X magnification, slides, coverslips, metal probes), Latex or nitrile gloves (soft rot bacteria are not infectious to humans, but do express a strong odor during infection), Potato, Sweet Potato and Cucumbers Begin with a large container for disinfecting the potatoes in 10% bleach and use the two drops of dish soap containing 10% bleach Use this solution to disinfect potato by placing the potato inside the large container After cleaning the potato with the solution go to your nearby sink and rinse it off and place the potato to the side Begin to put on you latex or nitrile gloves for this next part Grab your small beaker of bacterial inoculum and place it down in front of you to then grab the toothpick and dip it in the solution. Grab your potato once again and use the toothpick with the solution the the other hand to then insert it into the potato Once your done with that, use a damp paper towel to wrap around the potato and place it inside your sealable plastic bag Once you are done with the experiment you will repeat with 2 other vegetables (Sweet Potato and Cucumber) and wait for a week to see how your vegetables were affected, don’t forget to clean up after yourself At the end of the week you will use Microscopy equipment to see on a microscopy level on how the soft rot affected it
Investigation Theme PGST
Grade Level High School Students (Grades 9,10,11,12)
School Name Watsonville High School
Session Fall 2020

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NSF_Logo.jpg This material is based upon work supported by the National Science Foundation under Grant #2010556 and #1502892. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation.

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