Experimental Design |
Independent: We will inoculate the soft rot into the potato to see if it succumbs or fails to resist the soft rot.
Dependent: We will be measuring the plant detteriation from the soft rot we gave the potato as well as the other 2 vegetables.
Control: The potatoes that weren’t affected during the process of the inoculation of the soft rot.
Gather all your materials
Damp paper towels, Sealable plastic bags, Toothpicks, Small beakers of bacterial inoculum, A 10% bleach solution containing two drops of dish soap, A large container for disinfecting the potatoes in 10% bleach, Microscopy equipment (microscopes with 400X magnification, slides, coverslips, metal probes), Latex or nitrile gloves (soft rot bacteria are not infectious to humans, but do express a strong odor during infection), Potato, Sweet Potato and Cucumbers
Begin with a large container for disinfecting the potatoes in 10% bleach and use the two drops of dish soap containing 10% bleach
Use this solution to disinfect potato by placing the potato inside the large container
After cleaning the potato with the solution go to your nearby sink and rinse it off and place the potato to the side
Begin to put on you latex or nitrile gloves for this next part
Grab your small beaker of bacterial inoculum and place it down in front of you to then grab the toothpick and dip it in the solution.
Grab your potato once again and use the toothpick with the solution the the other hand to then insert it into the potato
Once your done with that, use a damp paper towel to wrap around the potato and place it inside your sealable plastic bag
Once you are done with the experiment you will repeat with 2 other vegetables (Sweet Potato and Cucumber) and wait for a week to see how your vegetables were affected, don’t forget to clean up after yourself
At the end of the week you will use Microscopy equipment to see on a microscopy level on how the soft rot affected it |