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Research on potato rot pathogens/bacteria:
Soft rot is caused by a range of bacteria around the world including - Pectobacterium carotovorum subspecies carotovorum (most common), Pectobacterium atrosepticum and Dickeya species, etc.
Dry rot is caused by fungal species in the genus Fusarium (thus the name Fusarium dry rot) The most important dry rot pathogen in the Northeast is Fusarium sambucinum, although Fusarium solani is also present.
Research on food rotting on the way to customers:
High concentration of ethylene causes premature aging and rotting of fruits and vegetables
Observations (qualitative) :
Day 1 & 2 - little to no change
Day 3 & 4 - grown tubers in the indents of the potato, white flakes around the inoculation site, circle dark patches throughout the potato without tubers, yellow color, dry and hard, not that rough of texture
Observations (quantitative) : Day 3 & 4: 8 tubers on the smallest potato, less tubers on medium potato (about 5) |
Research Question |
How does the type of damage affect potato rot? |
Predictions |
Greater the damage area, faster the potato rot |
Experimental Design |
Control: Regular potato (no cuts)
Length: 2 weeks
When to Collect Data: once every day
Independent variable: type of damage
Bruised ?
Punctured
Lesion
Partly skinned
Dependent variable: rotting (amount/quality)
Trials: Each person has 4 potatoes: one control potato & 3 trial potatoes (each person has a different cut)
Measurement: In centimeters
Materials:
plastic bags (same kind)
potatoes from the same pack
cutting tool (knife/others)
water
Qualitative type data:
smell
texture
color
solidity
tubers
patches on skin
where do signs of rot appear?
Quantitative type data:
# of tubers
# of patches on skin
How far did the rot spread from the damaged area?
PROCEDURE
1. Submerge in water ( 3 cups of water & 3 min).
2. Take out with tongs and place on a plate. Let dry for 5 minutes.
3. Cut/damage the potatoes.
4. Put directly into plastic bag + seal.
5. Repeat steps 1-4 for three trial potatoes per damage type.
6. Over a span of 2 weeks, check + record observations per day at 8:00 PM. |
Investigation Theme |
PGST |
Grade Level |
High School Students (Grades 9,10,11,12) |
School Name |
California Academy of Mathematics and Science |
Session |
Fall 2020 |