Planting Science - Projects: Bucket Listers
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Bucket Listers

Project by group 23sumf2colspr

Explore We know that there are different types of chlorophyll found in plants that utilize different wavelengths of light. Spinach leaves contain both chlorophyll an and chlorophyll b, which reflect yellow-green and green light respectively. We wonder what would happen if different wavelengths of light were used and how that would affect the rate of photosynthesis.
Research Question What is the effect of color of light on the rate of photosynthesis? Both Chlorophyll a and b are found in spinach. We know it absorbs and reflects different wavelengths of light. We want to test if that affects photosynthesis.
Predictions We know from our research that spinach contains Chlorophyll a and b. Both types reflect green and yellow light. It absorbs blue light best and red light second best. We predict that leaf disks receiving only green or yellow light will produce oxygen more slowly that those receiving blue or red spectrum of light.
Experimental Design Independent Variable: Color of light Dependent Variable: Time for leaf disk to float Materials: light filters (red, blue, yellow, green), 7 test tubes, spinach, paper punch, baking soda, distilled water, light source, syringe, small paintbrush, foil, soap solution 1. Wrap light filters around 5 test tubes. Wrap foil to create a dark tube, and leave one tube open to unfiltered light. 2. Create a beaker of 300 mL of distilled water with 6g baking soda added. Add 12 drops of soap solution. Divide it evenly between each of the 7 test tubes. 3. Punch 35 leaf disks out of the spinach with the paper punch. 4. Put 5 disks at a time into the syringe, with some of the distilled water. Apply a vacuum pressure until disks sink. Add to one of the tubes and then keep it in the dark. Repeat to fill all 7 tubes. 5. All at the same time, place the tubes in the light and start a timer. 6. Record the time each disk takes to float. 7. Collect data on how many disks are floating, every minute, for 30 min. At the end of 30 min, throw out data for any disks that were floating at 0 or still sunk at 30. Average the time of floating for the remaining disks for each tube.
Investigation Theme POS
Grade Level High School Students (Grades 9,10,11,12)
Teacher Name PlantingScience
School Name Digging Deeper {F2}
Session Summer 2023


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NSF_Logo.jpg This material is based upon work supported by the National Science Foundation under Grant #2010556 and #1502892. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation.

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