Research Interests (300 words)
My thesis work for my PhD is focused on the regulation of an Arabidopsis thaliana (a model plant related to mustard) transporter protein called AtMATE. This protein transports citrate, an organic acid that has many functions in plants, which include acting as a chelator, or binder, of ions. Citrate released into the soil can chelate aluminum, preventing it from entering the plant. This is important as aluminum is toxic to plants and causes reduced root growth. High aluminum is found in acidic soils, which includes 30% of the land on Earth. Much of the tropical and subtropical regions have acidic soil and thus, high aluminum. These regions often have food insecurity problems, so acid soil limitations to food production have a significant global impact. I want to understand AtMATE, and AtMATE regulation, to increase aluminum resistance in crop plants. My current work focuses on how a CBL/CIPK cascade affects the regulation of AtMATE. The CBL/CIPK cascade is a regulation system that causes changes in plant cells when the cells are stressed. I use assays in frog eggs to understand how the addition of the regulating proteins can cause AtMATE to function or to stop functioning. I also look at interactions and localization of the proteins in tobacco and Arabidopsis thaliana leaves, to understand how these proteins physically interact. I introduce my proteins of interest, linked to fluorescent tags, into plant cells to see where in the cell they localize. This work will provide the basis for the identification of similar regulation pathways in other species, with the ultimate goal of increasing aluminum resistance in crop plants.